ry- D2-3 line

Bill Engels wrengels at facstaff.wisc.edu
Wed Nov 8 21:44:56 EST 1995


In article <v01530501acc3bbe6b044@[142.150.128.166]>, j.dong at utoronto.ca
(Jianli Dong) wrote:

> I am looking for a ry- D2-3 containing line for microinjection. I am using
> ry as the P-element transformation marker.



Many D2-3(99B) lines are ry-, but since the D2-3 element itself carries
ry+ the flies have wildtype eyes. So those stocks are of no use to you. 

In fact, the D2-3 insertion at 99B has two copies of ry+ according to Hugh
Robertson. We and at least one other lab tried to get rid of the ry+
transgene by ordinary mutagenesis in order to make a stock for injecting
ry+ transposons, but we were not successful. We didn¹t know at the time
that there were two copies there! 

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William R. Engels                     :     WREngels at facstaff.wisc.edu
Genetics Department, 445 Henry Mall   :         office: (608) 263-2213
University of Wisconsin               :            lab: (608) 262-5578
Madison, WI  53706                    :            FAX: (608) 262-2976
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