Visible embryonic marker

rwilson rwilson at panda.uchc.edu
Tue Nov 14 16:44:02 EST 1995


  I've used x-gal to stain live embryos that had lacZ driven by a cut
enhancer element specific for the malpighian tubules. I dechorionated
and devitellinized them, then developed them for about 15 minutes. 
They survived for about 2 hours after that but could probably have
survived longer if I'd had changed the media or put em on a plate.



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