nuclear extracts

Kathleen Clark clark02 at UTSW.SWMED.EDU
Mon Oct 23 16:34:03 EST 1995


Dear Collegues,

   Has anyone tried to prepare nuclear extracts from Drosophila
ovaries/germ cells? It's my understanding that the nuclei are very fragile
and as such are hard to isolate intact. On the other hand, can anyone see a
problem with using unfractionated cellular protein for gel shift
experiments? Also, how much sucess have peple had at doing
immunolocalization on polytene chromosomes?  (i.e. looking at DNA binding
proteins)? Any feedback will be appreciated. 

Thanks!

Kathleen Clark
clark02 at utsw.swmed.edu





More information about the Dros mailing list