Plasmid rescue.

Ajay Srivastava ajays at sfu.ca
Mon Sep 11 16:13:33 EST 1995


Hello there,

I have been trying to do a plasmid rescue on a P-element line with the 
P-element of LacZ ry+ type having Kanamycin resistance(Karpen and 
Spradling Genetics 132, 737-753). Has anybody tried doing a rescue with 
insert of the above mentioned type, if yes then what concentration of 
kanamycin did you use?

Your help will be greatly appreciated.

Thanks,
Ajay.





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