genome size and library screening

Susan Glueck sg32 at postoffice.mail.cornell.edu
Thu Sep 14 09:44:44 EST 1995


The "ballpark" in our lab for screening genomic libraries of D.
melanogaster is to screen 1 x 10^6 plaques (one million) in order to get a
fair representative of the genome. What kind of numbers do you employ?

Furthemore, how much larger than Drosophila melanogaster is the genome of
Musca domestica? Then how many plaques would it be necessary to screen to
obtain a fair representation of that genome?

Susan Glueck
sg32 at cornell.edu



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