genome size and library screening

Martin Cann mjcann at med.cornell.edu
Fri Sep 15 14:34:54 EST 1995


In article <sg32-1409951047470001 at 132.236.171.240>,
sg32 at postoffice.mail.cornell.edu (Susan Glueck) wrote:

> The "ballpark" in our lab for screening genomic libraries of D.
> melanogaster is to screen 1 x 10^6 plaques (one million) in order to get a
> fair representative of the genome. What kind of numbers do you employ?
> 
> Furthemore, how much larger than Drosophila melanogaster is the genome of
> Musca domestica? Then how many plaques would it be necessary to screen to
> obtain a fair representation of that genome?
> 
> Susan Glueck
> sg32 at cornell.edu

I have been screening 1 x 10-6 with varying degrees of success.  So much
depends upon the library you use, how much it is age matched and the
relative expression of your gene of choice but I think it is a fair
figure.

Martin



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