Polytene Chromosome Squashes
storck-s at it.uwp.edu
Fri Sep 15 15:09:59 EST 1995
Hi! I am a graduate student at the University of Wisconsin - Parkside.
I have been following this newsgroup for about a month now, and I have
witnessed how helpful it can be to fellow Drosophila researchers.
I am wondering if anyone out there knows a good way to chromosome arm
separation when performing a squash of the salivary chromosomes. Right
now, I use a pencil to tap the chromosomes before squashing them firmly
with my thumbs, but when I view them under the microscope, they are too
bunched together, and therfore, I cannot see all the bands clearly. I am
following Protocol 17 in the Drosophila Laboratory Handbook by
Ashburner. Any help or suggestions in this process will be greatly
appreciated. Thank you.
Biological Sciences Graduate Department
University of Wisconsin - Parkside
PO Box 2000
Kenosha WI 53140
E-Mail: storck-s at it.uwp.edu
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