Polytene Chromosome Squashes

Ian Boussy iboussy at ORION.IT.LUC.EDU
Fri Sep 15 17:44:06 EST 1995


Denise,

You're in the same university system as one of the very best in the world 
at squashing chromosomes!  Contact Johng Lim at Eau Claire:

		Dr. Johng Lim
		Dept of Biology
		University of Wisconsin
		Park and Garfield
		Eau Claire, WI 54702-4004

		tel: (715) 836-3860
		FAX: (715) 836-2380
		email:  LIMJK at UWEC.EDU

I wouldn't presume to offer suggestions when you have the MASTER nearby! 
(And you can quote me!)

Ian

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Ian A. Boussy        			iboussy at orion.it.luc.edu
Associate Professor of Biology		  tel.  (312) 508-3635
Loyola University of Chicago		  FAX   (312) 508-3646
 >< >< >< >< >< >< >< >< >< >< >< >< >< >< >< >< >< >< >< >< 

On 15 Sep 1995, Denise Storck-schattner wrote:

> Hi!  I am a graduate student at the University of Wisconsin - Parkside.  
> I have been following this newsgroup for about a month now, and I have 
> witnessed how helpful it can be to fellow Drosophila researchers. 
> 
> I am wondering if anyone out there knows a good way to chromosome arm 
> separation when performing a squash of the salivary chromosomes.  Right 
> now, I use a pencil to tap the chromosomes before squashing them firmly 
> with my thumbs, but when I view them under the microscope, they are too 
> bunched together, and therfore, I cannot see all the bands clearly.  I am 
> following Protocol 17 in the Drosophila Laboratory Handbook by 
> Ashburner.  Any help or suggestions in this process will be greatly 
> appreciated.  Thank you.
> 
> Denise Schattner
> Biological Sciences Graduate Department
> University of Wisconsin - Parkside
> PO Box 2000
> Kenosha WI  53140
> 414-595-2707
> E-Mail:  storck-s at it.uwp.edu
> 
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