tags for subcellular localisation in flies
bs1 at mrc-lmb.cam.ac.uk
Mon Aug 5 13:39:45 EST 1996
I need to tag transmb constructs on the intracellular domain, and I
want a good tag for doing westerns, immunoprecipitations and more
importantly, look at the intracellular localisation of my constructs.
Does anybody know a tag that will work well for these kind of
experiments? The tags that I can think of at the moment are Flu, Flag,
HA, Strep and T7 (I would prefer not to use the myc-tag) they all seem
to work well in vitro, but nobody around me knows about how they work
for subcellular localisation in flies...
thanks in advance,
More information about the Dros