heat shock protocol
Martin E. Feder
m-feder at uchicago.edu
Wed Aug 7 09:00:15 EST 1996
Except in very early embryos (< 6 hr), 1 hour at 36 C should yield a
robust heat shock response. 34 is too low, and 38 is too high.
Typically we moisten a filter paper circle in PBS, place it in a small
disposable petri dish, put the larvae or pupae on the filter paper,
cover the dish, and seal it tightly with Parafilm. The entire dish is
then immersed in a thermostatted water bath for the specified time.
Email me for more details if this is not sufficient.
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