heat shock protocol

[Martin E. Feder]

Except in very early embryos (< 6 hr), 1 hour at 36 C should yield a 
robust heat shock response.  34 is too low, and 38 is too high.  
Typically we moisten a filter paper circle in PBS, place it in a small 
disposable petri dish, put the larvae or pupae on the filter paper, 
cover the dish, and seal it tightly with Parafilm.  The entire dish is 
then immersed in a thermostatted water bath for the specified time.  
Email me for more details if this is not sufficient.