Drosophila neuron membranes

[dhodges at UCI.EDU]

Does anyone have  references or first hand information regarding the lipid
content of Drosophila cells, in particular neurons?  I have attempted to
permeabilize cultured embryonic neurons using a variety of lipids  and
streptolysin O, which is known to act via cholesterol in the membrane.  I
have seen no evidence of uptake of a fluorescent reporter using either
method at a variety of concentrations and under many different conditions.
I am beginning to believe that fly membranes are drastically different from
mammalian cell membranes.  Any advice would be greatly appreciated.

Thanks,

Dianne Hodges
UC Irvine
Dept. of Anatomy & Neurobiology
Irvine, CA 92697
(714) 824-8141