epitope tagging for ovary detection
forte at ohsu.edu
Mon Feb 26 18:30:51 EST 1996
In article <4gofqg$49r at nntp3.u.washington.edu>, dgilles at u.washington.edu
(Doreen Gillespie) wrote:
> I am looking into possible epitopes to use for tagging a protein
> expressed in the germline. Does anyone have suggestions, experience,
> epitopes to avoid? My understanding is that myc tags have been extremely
> variable, due in part to poor antibodies. Has anyone tried the
> hemaglutinin epitope? One of my key concerns is high background--the
> 9E10 myc antibody isn't fantastic, and I don't know about the
> hemaglutinin antibody (BAbCO).
> Thanks for any thoughts,
> Doreen Gillespie
We have had good luck using the HA antibody (12CA5; Boehringer) in flies.
Although we have not looked a lots of tissues, it certainly works cleanly
in imaginal discs and pupal wings.
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