transgenics with big constructs

Dipak Mahato dmahato at unity.ncsu.edu
Thu Feb 29 10:24:46 EST 1996


Hi,

I will soon be trying to make transgenics with clones from John Tamkun's 
p-element cosmid library.  These are very big clones (35kb insert/12kb 
vector).  If anyone has any experience making transgenics with clones 
this big, or from this library, I would appreciate any advice (like what 
concentration of DNA to use, what injection buffer, use of helper 
plasmids...).  

Thanks,
Dipak Mahato




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