Any Qiagen RNeasy users out there?

[Jean Leloup]

In article <jreiss-2401961804280001 at pelican.biology.emory.edu>,
jreiss at biology.emory.edu (Jonathan A. Reiss) wrote:

>    
> 
> I am interested in using this system for production of small amounts of
> total rna for rt-pcr experiments on 100-200 embryos.  it's obvious that
> the key step is getting a good homogenate. 
> 
> help/suggestions/caveats?
> 
> thanks.
> 
> jonathan reiss
> 
> jreiss at biology.emory.edu

Watch out, chloride inhibits reverse transcriptase (see in Maniatis).