Help : K currents from Drosophila embryonic neurons
gupta at acsu.buffalo.edu
Wed Mar 6 16:56:15 EST 1996
I am recording potassium currents from Drosophila embryonic neurons, and
have some technical queries.
Is it easy to go into the whole cell mode of voltage clamp without
getting any leak currents? I find that the probability of getting a good
current is very low in this system. I want to know if this generally
happens while recording potassium currents from this system.
Can anyone tell me the success rate of recording potassium currents from
IS observing a high leak common in these cells?
How long can the currents be recorded from these cells in the whole cell
Can anyone send me a good embryonic culture protocol?
IS there a procedure to obtain isolated neurons other than using
cytochalasin/chemically enriched medium?
Your help will be greatly appreciated.
I am posting this from a friend's account so could you please reply
to me at khanna at acsu.buffalo.edu
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