Help : K currents from Drosophila embryonic neurons

[Vijay Gupta]

Hi ! 

I am recording potassium currents from Drosophila embryonic neurons, and 
have some technical queries. 

Is it easy to go into the whole cell mode of voltage clamp without 
getting any leak currents? I find that the probability of getting a good 
current is very low in this system. I want to know if this generally 
happens while recording potassium currents from this system. 

Can anyone tell me the success rate of recording potassium currents from 
these neurons?

IS observing a high leak common in these cells? 

How long can the currents be recorded from these cells in the whole cell 
mode?

Can anyone send me a good embryonic culture protocol?

IS there a procedure to obtain isolated neurons other than using 
cytochalasin/chemically enriched medium?

Your help will be greatly appreciated.

I am posting this from a friend's account so could you please reply
to me at khanna at acsu.buffalo.edu

Thanks

Bye
Ashish