RP49 as a control in RT-PCR?
lmjnutte at ACS.UCALGARY.CA
Sat Mar 9 01:31:12 EST 1996
I want to use RP49 as a control in RT-PCR to test for cDNA quality. Has
anyone used this before? If so, I would really appreciate the sequence
of the primers that you used along with the PCR conditions. If not,
what have people been using out there? And again, I would appreciate
the relevant sequence and reaction conditions. Given my previous post,
something else may be better for RT-PCR on testis RNA???
By the way, no I don't want this to be quantitative PCR, I just want to
be able to judge RNA/cDNA quality.
Thanks for your help.
Dept. Biological Sciences
The University of Calgary
lmjnutte at acs.ucalgary.ca
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