leakiness in UAS system?
sprenger at CGL.UCSF.EDU
Thu Mar 21 18:26:02 EST 1996
At 8:29 AM 3/21/96, Dale A Dickinson wrote:
>Our lab has some preliminary results which suggest that UAS-fussion
>constructs are leaky. I have read a lot about this system, and haven't
>come across any reports that suggest leakiness. Does anyone out there
>know if this has been observed before? It is quite important that the
>lines I construct do not express until crossed to gal4 lines.
>Please feel free to email me directly,
>Dept. of Molecular Biology and Genetics,
>University of Guelph
>Guelph, ON Canada
>dadickin at uoguelph.ca
We also observe leakiness using the pUAST vector that interestingly is
temperature dependent. At 17 C I don't see any problems but at 25 C all the
flies die because of leaky expression of the transgene.
San Francisco, CA 94122
sprenger at cgl.ucsf.edu
More information about the Dros