Single embryo PCR

[a]

In article <323734CE.66F0 at u.arizona.edu>, rnarayan at u.arizona.edu wrote:

> I'm looking for a protocol describing the amplification of genomic DNA
> from a single embryo as my homozygous flies are embryonic lethals.  Can
> anyone help?


Alan Christensen had a note about this in DIS and DIN. Here are the
FlyBase Reference Reports:


Title       Preparation of DNA from single embryos for PCR.
Author(s)   Garozzo, M.
            Christensen, A.C.
Year        1994
Journal     D. I. N.
Volume      13
Flybase ID  FBrf0082018   Look up ref in Genes, Aberrations, Clones
Also in     FBrf0082019




Title       Preparation of DNA from single embryos for PCR.
Author(s)   Garozzo, M.
            Christensen, A.C.
Year        1994
Journal     D. I. S.
Volume      75
Pages       204--205
Coden       DRISAA
Flybase ID  FBrf0082019   Look up ref in Genes, Aberrations, Clones
Also in     FBrf0082018

You can find the complete text on FlyBase.


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