anti HRP and anti B-gal - any recommendations?
Emma Rushton
rushton at biology.utah.edu
Wed Sep 18 21:25:16 EST 1996
I need to buy some anti betagalactosidase and anti horse radish peroxidase.
This is what I have found out so far:
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rabbit anti b-gal from Cappel is $215 for 2 ml. Dilution 1/10,000 works
well in
my experience
goat anti b-gal from Arnel is $30 for 2 ml They suggest dilution
1/25,000
(New York)
mouse anti b-gal from Sigma is $91 for 2 ml No dilution suggested.
Note the enormous price differences! Sigma references Frasch 1995 for the
use of their anti b-gal on Drosophila.
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rabbit anti HRP from Cappel is $44 for 2 ml
or $151 for 5 ml of IgG
goat anti HRP from Cappel is $44 for 2 ml
or $145 for 5 ml of IgG
rabbit anti HRP from Sigma is $25.10 for 1 ml
or $41.80 for 2 ml
goat anti HRP from Sigma is $22.90 for 1 ml
or $38.15 for 2 ml
Sigma references Gorozyca 1994 for the use of their goat anti HRP on
Drosophila.
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Has anyone any good ar bad experience of any of these antibodies? I am of
course very interested in the Arnel anti b-gal, but I have a nasty
suspicious mind, and I'm too tight-fisted to try an antibody without asking
for the fruits of others' experience first!
If I find anything out, I'll publish it here.
Emma
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