anti HRP and anti B-gal - any recommendations?

Emma Rushton rushton at biology.utah.edu
Wed Sep 18 21:25:16 EST 1996


I need to buy some anti betagalactosidase and anti horse radish peroxidase.
 This is what I have found out so far:

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rabbit anti b-gal from Cappel is $215 for 2 ml.  Dilution 1/10,000 works
well in 
																																																	my experience

goat   anti b-gal from Arnel  is $30  for 2 ml   They suggest dilution
1/25,000
															   (New York)   

mouse  anti b-gal from Sigma  is $91  for 2 ml   No dilution suggested.


Note the enormous price differences!  Sigma references Frasch 1995 for the
use of their anti b-gal on Drosophila.

--------------------------------------------------------------------------------

rabbit anti HRP from Cappel is $44 for 2 ml 
																												or $151 for 5 ml of IgG

goat   anti HRP from Cappel is $44 for 2 ml
                            or $145 for 5 ml of IgG

rabbit anti HRP from Sigma is $25.10 for 1 ml
                           or $41.80 for 2 ml

goat   anti HRP from Sigma is $22.90 for 1 ml
                           or $38.15 for 2 ml 

Sigma references Gorozyca 1994 for the use of their goat anti HRP on
Drosophila.

--------------------------------------------------------------------------------

Has anyone any good ar bad experience of any of these antibodies?  I am of
course very interested in the Arnel anti b-gal, but I have a nasty
suspicious mind, and I'm too tight-fisted to try an antibody without asking
for the fruits of others' experience first!

If I find anything out, I'll publish it here.

Emma



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