Drosophila genomic southerns
Richard M Kliman
rkliman at runet.edu
Wed Apr 9 12:22:08 EST 1997
In article <97097.000239VHWCC at CUNYVM.CUNY.EDU>,
Harshinee Wijesinghe <VHWCC at CUNYVM.CUNY.EDU> wrote:
>Could somebody tell me an effective, non-radioactive labelling method
>that can be used for the probe in Drosophila genomic southerns? Thanks
>in advance for any help.
We did genomic Southerns in a molecular biology course this year. We
used an actin probe derived from Dictyostelium to detect homologoues in
D. melanogaster. Dicty actin-8 PCR was labelled with DIG-UTP by random
priming using the Genius kit. Using the color reactions (not the
chemilumiscent reaction), we were able to clearly detect 6 bands in a D.
melanogaster HindIII digest. I was surprised that this method worked
with a genome the size of Drosophila, but it did.
Dept. of Biology
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