Xgal embryo stain without fixation

Gerard Manning ger at stanford.edu
Sat Aug 16 21:31:42 EST 1997


I've heard that it's possible to stain embryos with Xgal without fixation,
in order select homozygous mutants away from blue balanced siblings, for
PCR sequencing. Does anybody have any details of how it can be done? (I
haven't yet tried, but I would imagine that it would require
permeabilisation or removal of the vitelline membrane).

   Thanks!

        -Gerard Manning.



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