plasmid rescue from both sides of PZ

Marc Champagne M.Champagne at nospam.cellbio.duke.edu
Tue Aug 26 07:31:32 EST 1997


In article <v01540b02b025ef4480d9@[140.141.8.58]>, liebl at CC.DENISON.EDU
(Eric C. Liebl) wrote:

> Does anyone know of a restriction enzyme combination that will allow me to
> rescue genomic DNA from the "rosy gene side" of a PZ insert?  In
> otherwords, is there a way to rescue genomic DNA on the opposite side of
> that rescued by XbaI cutting?
> 
> Thanks, ERIC

I got a protocol off of Flybase doing exactly that.  Look for PZ at
http://morgan.harvard.edu

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