plasmid rescue from both sides of PZ

Marc Champagne M.Champagne at
Tue Aug 26 07:37:59 EST 1997

In article <M.Champagne-2608971331320001 at>,
M.Champagne at (Marc Champagne) wrote:

> In article <v01540b02b025ef4480d9@[]>, liebl at CC.DENISON.EDU
> (Eric C. Liebl) wrote:
> > Does anyone know of a restriction enzyme combination that will allow me to
> > rescue genomic DNA from the "rosy gene side" of a PZ insert?  In
> > otherwords, is there a way to rescue genomic DNA on the opposite side of
> > that rescued by XbaI cutting?
> > 
> > Thanks, ERIC
> I got a protocol off of Flybase doing exactly that.  Look for PZ at

Oops, i actually found a very detailed protocol (by J.Rehm) at the BDGP site:


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