plasmid rescue from both sides of PZ

[Marc Champagne]

In article <M.Champagne-2608971331320001 at ocarina.cellbio.duke.edu>,
M.Champagne at nospam.cellbio.duke.edu (Marc Champagne) wrote:

> In article <v01540b02b025ef4480d9@[140.141.8.58]>, liebl at CC.DENISON.EDU
> (Eric C. Liebl) wrote:
> 
> > Does anyone know of a restriction enzyme combination that will allow me to
> > rescue genomic DNA from the "rosy gene side" of a PZ insert?  In
> > otherwords, is there a way to rescue genomic DNA on the opposite side of
> > that rescued by XbaI cutting?
> > 
> > Thanks, ERIC
> 
> I got a protocol off of Flybase doing exactly that.  Look for PZ at
> http://morgan.harvard.edu
> 

Oops, i actually found a very detailed protocol (by J.Rehm) at the BDGP site:

http://fruitfly.berkeley.edu/BDGP/inverse_pcr.html

m

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