X-gal/In situ protocol

Benjamin T. Kehl kehl at bcm.tmc.edu
Tue Dec 2 10:52:26 EST 1997


I'm looking for a good protocol for X-gal/in situ double labeling for
3rd instar larvae.  I have tried one which did the X-gal staining first,
followed by in situ, but the insitu did not work.  Alternatively, I've
also tried doing the insitu first followed by anti-bgal antibody
staining.  It is very hard to distinguish between the signals and I had
some problems with background.  If anybody has any suggestions, I would
greatly appreciate them!

Thanks!

Benjamin T. Kehl
kehl at bcm.tmc.edu
Baylor College of Medicine
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