Xgal staining

Lertluk Ngernsiri etlngern at DINGO.CC.UQ.EDU.AU
Tue Jul 1 21:06:55 EST 1997

Dear Drosophilists,
I am carrying out a screen on embryos with a PlacZ background. I am trying
to optimise the Xgal staining of fixed, undevitellinized embryos. To date I
have had most success with Ghysen and O'Kane's method, and no luck at all
using XGal dissolved in dimethylsulfoxide. Would experienced workers be
willing to share details of their successful protocols?

Lertluk Ngernsiri                 Ph:  +61 7 3365 7469
Department of Entomology          Fax: +61 7 3365 1922
University of Queensland          e-mail:L.Ngernsiri at mailbox.uq.edu.au
Brisbane Q 4072

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