Oxford PhD studentship - Drosophila cell cycle
myles.axton at zoo.ox.ac.uk
Sun Jun 15 07:56:03 EST 1997
MRC graduate studentship for October '97
Genes regulating DNA replication at fertilisation.
Genetic interaction of maternally-acting mutations identify regulators of
the Plu inhibitor of DNA replication in Drosophila embryos (Elfring et al.
1997 Mol. Biol. of the Cell. 8, 583-593). It is not currently known how
Plu functions, but it bears some resemblance to the tumour suppressor
protein p16INK4. cDNAs encoding Plu-binding proteins have been isolated by
a yeast 2-hybrid screen. This project would use the genetics, molecular
biology and cytology of Drosophila to identify the roles of these
proteins in S-phase regulation.
Genome stability and DNA mismatch repair in Drosophila.
Mutagen sensitive and meiotic mutants will be screened for microsatellite
replication errors using a PCR assay. Replication fidelity defects will be
further characterised by a modified Kunkel assay (Umar et al. 1994.
Science 266, 814-816) involving microinjection of heteroduplex templates
into embryos from mutant flies. The developmental requirement for a
recently cloned mismatch repair gene will be examined through genetic
analysis of mutants.
Candidates should be UK or EU residents who have obtained or are expecting
a 2.i honours degree in biology, genetics or biochemistry. The studentship
pays a 3-year stipend (UK residents only) plus University and College fees
(UK and EU residents). You would be working in a group with interests in
the cell cycle, growth and animal development and you would interact with
members of the Molecular Evolution groups. Send a CV and the names of 2
Please immediately contact:
Dr J. Myles Axton
University of Oxford
Department of Zoology
South Parks Rd.
Oxford OX1 3PS
Telephone 44 (0) 1865 557431
Fax 44 (0) 1865 271228
Email myles.axton at zoo.ox.ac.uk
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