EMS mutagenesis hit ratio formula

ME me at ucdavis.edu
Fri Jun 20 20:10:12 EST 1997


Can anyone provide a reference and/or formula for calculating the efficiency
of EMS mutagenesis, based on mutation rate, genome size, etc.  I.e., what
mutation rate/EMS conc. is needed to get a 95% confidence of hitting every
gene atleast once, as in the Nusslein-Volhard Wieschaus screen?  This is not
for Drosophila, so I would like a variable parameter for genome size.



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