P1 Filters

[Greg Roman]

Mike,

   We have been using the P1 filters to map a few genes.  We have found
clear hybridization to mapped P1s in each case.  As long as you check the
P1 for colinearity with genomic DNA on a Southern, the mapped P1 gives you
a pretty precise location. I am not aware of the actual statistic, but it
appears the majority of P1s have been mapped directly or anchored to a
mapped contig. Much of the genome seems to be covered by these contigs.
I'll be glad to answer any other specific questions you may have about the
use of these filters; just drop me an email. 
    Perhaps this is a good place to reccommmend that the BDGP develop a
way to recieve copies of these hybridizations to incorporate into the P1
project. They could evalute the quality of these data and include them in
the P1 map.  Depending on the number of people using these filters, this
could rapidily anchor most of the floating P1s and refine the exisiting
contigs even further.     


Gregg Roman
Department of Cell Biology
Baylor College of Medicine 
Houston,  Tx 77030
roman at bcm.tmc.edu


In article <forte-2005971655250001 at 137.53.98.58>, forte at ohsu.edu (Michael
Forte) wrote:

> Hi,
> 
> I'd be interested hearing from folks who have used the fly P1 filters
> available from Genome Systems.  Are these any good and have they served as
> a useful alternative to good old chromosome squashes?
> 
> Mike Forte