Immuno on embryos-update

Martin Cann mjcann at MED.CORNELL.EDU
Thu Apr 23 15:02:10 EST 1998


Many thanks to everyone for the advice.  The consensus appears to be:

1)  After harvesting and popping vitelline membranes, destroy endogenous
HRP with a H2O2/MeOH step (3% 15 mins).

2)  Use fresh DAB made from powder rather than the premade Vector
stuff.  This can lower background.

3)  Ni2+ can increase background.  Avoid if possible.

Martin



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