Double labelling of embryos

Jon Clayton JDC12 at le.ac.uk
Fri Jun 12 16:58:45 EST 1998


Does anyone know of a protocol suitable for double labelling embryos
with both anti-(Dig labelled riboprobe) and anti-(lacZ)? I'd like to
look at the expression pattern of an embryonic lethal gene in
homozygotes. I have a stock balanced with TM3, hb::lacZ and I plan to
discard those embryos giving a lacZ signal. Or is there a better way to
do this? Many thanks
	jdc12 at le.ac.uk



More information about the Dros mailing list