FLAG Tagged Proteins

Michael Forte forte at ohsu.edu
Mon Mar 9 14:58:25 EST 1998


Hi,

We have recently made some transformed lines which express FLAG-tagged
proteins (FLAG is at the extreme N terminus).  Although we get phenotypes,
we are having trouble detecting protein expression immunocytochemcially
using several copmmercial FLAG antibodies.  Anyone ever get FLAG
antibodies to work immunocytochemically and if so, were there tricks
(i.e., fixation conditions, specific antibody source).  Thanks for any
help.

Mike Forte



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