RT PCR

[Claire Russell]

Dear All,
I'm thinking of doing an expt where I fix and X gal stain larvae before 1st
strand cDNA synthesis and PCR. Has anyone tried this? Will the fixing
inhibit the reactions?
My other question about RT PCR is how few larvae could I do it on?

Any help will be greatly appreciated
Thanks
Claire

Dr Claire Russell
Dept of Biochemistry
Imperial College
Exhibition Road
London
SW7 2AZ
UK

Tel  +171 594 5306
Fax  +171 225 0960
e-mail  c.russell at ic.ac.uk