study of a region

[Kathleen Gajewski]

Well, I can answer the first question regarding cosmids: yes it can be 
done, but it's not very efficient.  I have successfully transformed with 
2 clones from John Tamkum's NotBamNot-CoSpeR library, and the inserts ran 
about 40 kb.  If you do inject cosmids, be prepared for difficulties.  
The needles have a greater tendency to clog, and you must be prepared to 
set up several hundred (at least!) G zero matings.  And this is assuming 
that someone experienced is doing the injecting.