P element screen
kaufman at bio.indiana.edu
Sun Apr 11 13:14:07 EST 1999
As an adjunct to the Drosophila genome sequencing effort we are planning to
generate tens of thousands of P-element insertions in D. melanogaster to
obtain single P-element insertions in the vast majority of genes. Based
on the analysis of many enhancer detector screens by the BDGP, we surmise
that a P-element can be obtained in 75% to 95% of all the Drosophila genes
(viables and lethals). The basic strategy is to generate single P-element
insertions throughout the genome, sequence the adjacent genomic DNA, and
compare the sequence (STS) with existing genomic sequences and ESTs. We
will keep those P-elements that are inserted in proximity of a gene: e.g.,
in the ORF, in the 5'UTR, or within a few hundred bases of the 5'UTR.
These P-element stocks will then be deposited in the Stock Center in
We are planning on using simple P-elements that contain the white[+] gene.
Three different P-elements are at present being considered:
1) A P-element that contains an enhancer detector driving GAL4 (e.g., Brand
and Perrimon, 1993)
2) 2) A P-element that contains UAS sites in front of a promoter to
facilitate ectopic expression screens (e.g., Rorth, 1997)
3) 3) A P-element containing FRT sites.
We welcome your input and thoughts as we believe that this is a one time
experiment given the magnitude of the task. If you have any suggestions,
please contact hbellen at bcm.tmc.edu before May 1, 1999. Thank You.
Thom Kaufman kaufman at sunflower.bio.indiana.edu
--- Biology Dept., Indiana University, Bloomington, IN 47405 ---
More information about the Dros