Does anyone know if I need to linearize pRMHA3 prior to transfection into drosphila cells? I'm not into fly research and am using the vector along with phshsneo-2 for expression of recobinant proteins. I have a horrible map of it and haven't any idea if the cloned gene to be expressed lies within a transposible element. Can anybody help? Please respond to rcrawl at vmmc.org THANKS