in situ - tracheal staining - artifact?

siaumin siauminf at
Fri Nov 12 18:13:23 EST 1999

I have tried performing RNA in situ hybridization on drosphila embryos
for the first time using both antisense and sense probes. Both trials
have resulted in tracheal staining (in the older embryos) that I fear
may be artifactual.  I was wondering if anyone has run into this problem
before or knows what could cause such a result.  Any advice would be
greatly appreciated.
thank you. 

Please Respond to Karen at kartiles at

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