help

liurainb at 263.NET liurainb at 263.NET
Tue Nov 30 02:21:12 EST 1999


Dear Prof:
     This letter comes from Cardiovascular Institute, Fuwai Hospital, Beijing, China. We are interested in Drosophila, because we have two strains related to anesthetic. We want to screen the special genes from these two strains. But now, I have a problem of RNA extraction from Drosophila adult, that I only see two bands (18s, 5s) on the normal agarose(0.8), but on the agarose-formaldehyde gel, there are 28s and 5s bands, no 18s bands after the RNA extraction using TRIzol reagent. I cannot understand about this. So I would like to ask you how many bands you have seen on the gel when you done the RNA extraction. Thank you.
     Would you like to give me the protocol of the Drosophila RNA extraction and somes advices about the Drosophila RNA and the photo when you have time. I will be very happy if I could receive your letter. Thank you very much!
     I found the mail address from Ceolas.
     I am looking forwards to your reply!
Your sincerely,

Hong Liu


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