Help with TM6 phenotype

[Chris Jones & Deirdre Sumpter]

In article <380784B1.7EC82543 at helix.nih.gov>, jbanders at helix.nih.gov wrote:

> I want to do a crossing scheme where at one point I will need to be able
> to distinguish Sb/+ from Sb/TM6. I know that TM6 is marked with
> Ubx(bx34e) but I have no experience with this marker. My question is:
> how hard is it to distinguish the phenotype of Ubx and how reliable is
> the phenotype (I'm growing the flies on molasses food at 25 degrees). Is
> the identification reliable for the naive or should I design the
> experiment another way?

John --

IME, scoring Ubx is not terribly reliable (though depending on how many
animals you need, it may be good enough for you). Hn is probably on your
TM6, but I've never used it. I suggest using TM6B instead of TM6 if you
can: Tb can be scored in the pupae, and Hu in the adults if need be. Good
luck,

Chirs Jones

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