Pelement

[ali cyrus ravanpay]

Hi--

I've been trying to isolate sequences flanking a PlacW element, which I
believe resides in the X chromosome centromeric heterochromatin.  I've
used the
"inverse PCR protocol" (digesting genomic DNA, ligating and using inverse
PCR primers), with no success (positive control works!).  I am wondering
whether this is due to the centromeric heterochromatin that flanks the
pelement.  Has anyone successfuly rescued a pelement from heterochromatin,
if yes, which method was used?

Sincerely appreciate your response, 
Ali

 Ali Cyrus Ravanpay
 University of Washington
 MSTP MedI


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