Request for two Dp stocks

[Kevin Cook]

Hi Hong--

The Bloomington Stock Center maintains a duplication with the same 
breakpoints as Dp(1;Y)g[+].  It is Dp(1;f)LJ9, in stock

3219	C(1;Y)1, Df(1)g, y[1] f[1] B[1]/C(1)A, y[1]/Dp(1;f)LJ9, y[+] g[+] 
na[+] Ste[+],	

which duplicates 12A6-10;13A2-5 and the tip of the X (1A1;1B3-4). The 
12A;13A region is subdivided by Dp(1;f)LJ8, in stock

2870	C(1;Y)1, y[1] g[2] na[1]/FM3/Dp(1;f)LJ8, y[+] g[+] na[+] Ste[+],

which duplicates 12E1;13A2-5 (and perhaps a bit more distally) and the same 
X tip region.

These two duplications were derived from In(1)sc[29] (1B3-4;13A2-5) by 
deleting everything between the distal bands and the centric 
heterochromatin.  Dp(1;Y)g[+] and Dp(1;Y)na[+] were probably made the same 
way, except that In(1)sc[29] was first crossed onto the attached XY 
chromosome C(1;Y)6 (also known as "XYL.YS").  Irradiating a In(1)sc[29] + 
C(1;Y)6 chromosome would be an efficient way to make a series of Dp(1;Y) 
chromosomes if you need to duplication map your mutation more closely.  You 
can read a really nice description of a screen like this in a DIS article 
from Juan Botas and Brenda Lilly ("An efficient mutagenesis screen to 
generate duplications of polytene section 8 on the X chromosome of 
Drosophila melanogaster", D. I. S. 1999 82:105--112).  You can also look at 
http://flybase.bio.indiana.edu/.bin/fbpcq.html?FBrf0125111 for a brief 
description of a screen we did.

Kevin
__________________________________________________________
Kevin Cook, Ph.D.		Bloomington Drosophila Stock Center
Department of Biology		http://flystocks.bio.indiana.edu
Jordan Hall 142		
Indiana University		812-855-5782
1001 E. Third St.		812-855-2577 (fax)
Bloomington, IN  47405-3700	kcook at bio.indiana.edu


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