Enquiry regarding competent cells for transforming the insect vector PUAST

Soma Ganguly soma at ncbs.res.in
Tue Dec 26 16:49:31 EST 2000


HI,
Could you please suggest some good bacterial strains which can transform
the plasmid  PUAST efficiently?
I have been using HB 101  strain till now.But the problem wiht HB 101
strain is
that it is methylating the restriction sites and the digestions are not
working.Is there any remedy to avoid this?PLease suggest.
Thank you
  Soma.
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Soma Ganguly
Junior Research Fellow
Prof.VijayRaghavan's Lab
National Centre for Biological Sciences
Tata Institute of Fundamental Research
UAS Campus,GKVK.
Bangalore:5600 65.
India.
e-mail:soma at ncbs.res.in
       soma.ganguly at usa.net
Tel: 91-080-3636420/21/22/24/25/26
       Extn: 2241/2242
Fax: 91-080-3636662  


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