bstrub at uoguelph.ca
Mon Feb 7 02:12:29 EST 2000
Several options are available for Drosophila mutagenesis.
A common one is EMS (ethyl methanesulfonate) mutagenesis, in which male flies
are fed on this chemical, and then mated. The procedure is relatively safe.
See Lewis and Bacher, DIS 43:193 (1968).
Also, you could try P-element insertional mutagenesis. This is less random, as
some sites cannot accommodate insertion of a P-element. However, any mutations
that are produced can be analyzed by molecular techniques, and this can be
quite beneficial. See Cooley et al., Science 239:1121 (1988).
University of Guelph
Guelph, Ontario, Canada
Alan Meyer wrote:
> I am a college student performing my first research experiment--mutagenesis
> of flies. I have all of my cultures and would like to know if anyone can
> help me find a relatively easy mutagen to use in my work. I'm really quite
> the amateur at this whole thing.
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