gopalg at uclink4.berkeley.edu
Fri Jul 21 09:34:15 EST 2000
I am trying to sequence a 80 kb long P1 insert.When i use intact plasmid, i find it difficult to get a good sequence. Is there any good wayto do the sequencing,otherthan shotgun subcloning, to walk a P1 insert?
Please email me back your replies, since i donot subscribe to this mailing list.
Thanks for all your time,
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