ProteinA tagging in transgenes?

S. Findley polliwog at u.washington.edu
Tue May 30 12:27:12 EST 2000


Hey...has anybody out there tried expressing Protein A-tagged versions of
transgenes in bugs? I am interested in utilizing this tag for
affinity-purification of protein complexes from squished-up bug ovaries.
Just curious if there is enough background binding to warrant a negative
control (PrtA-only) Transgenic.


Thanks

-SDF
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Seth D. Findley
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