Sutter P-97

[Laszlo Sipos]

Dear Colleagues,

I would be very grateful if someone could help me in microinjection
needle pulling with Sutter P-97. The parameters

01 Heat=700  Pull=20  Vel=18  Time=80
02 Heat=640  Pull=100 Vel=15 Time=? (not listed)

which I used successfully on P-80PC burns the filament trying to
use capillaries (Sutter):

borosilicate with filament
o.d.: 1.0mm, I.D.:0.50mm
10 cm length
catalog#: BF100-50-10

What kind of filament/glass should I try to use???

Many thanks in advance!

Laszlo Sipos

Hungarian Academy of Sciences
Biological Research Center,
Institute of Genetics
H-6701 Szeged
Hungary
email: Sipos at nucleus.szbk.u-szeged.hu


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