RNA in situs

Moran, C.N. cnm5 at leicester.ac.uk
Fri Nov 17 14:03:12 EST 2000


Dear All,

I've a lot of RNA in situs planned and thought about synthesising primers
with either the T3 or T7 pormotor tacked onto them.  Can anyone tell me the
minimum amount of promotor sequence I need to use?  And do I need a spacer?

Thanks.

Colin.

Colin Moran
Lab 136, Adrian Building
Genetics Department
Leicester University
University Road
LE1 7RH
Tel: +44 (0)116 252 3421
 
cnm5 at le.ac.uk <mailto:cnm5 at le.ac.uk>
 


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