Ligatio

Aramia Asmar aramia at its.caltech.edu
Sat Nov 18 19:59:22 EST 2000


I need to amplify a large DNA fragment about 20kbp, so to obtain that I
have tried to use PCR and amplify this fragment using taqplus but it
doesn't work that well.
I tried to amplify two 10kbp fragments instead and ligate it...but as my
fragments are two large the ligation doesn't work either....shall I try
cloning instead and what king of vectors should I use for cloning of such
big fragments.
Would be grateful for any help


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