Embryo in situ problems

Cathy Slack cathy.slack at ic.ac.uk
Mon Aug 6 08:04:09 EST 2001

I have been having problems with in situ hybrisisation to embryos using
DIG-labelled RNA probes. I basically see a lot of non-specific labelling
and even see the probe hybridising to the nucleus! I don't think its my
probe as different probes give the same result. Has anyone ever
experienced this problem before and if so how do I rectify it??? I am
basically following a standard in situ protocol.


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