why do i not get transformants

Sri srikanth951124 at yahoo.com
Tue Jan 9 17:04:13 EST 2001


Hi,

I got a few responswes -al asking for some more detail
- here is the detail

the problem that i have is as follows:
i have made a fusion of a protein with gfp and have
injected into KPD
embryos of Drosophila melanogaster.. the dna is at a
concentration of
around 500ng/ul. after
injections i have set up about 100 lines of hte
injectedlarvae and i do
not get any transformants.. i do not know the reason
for hte same.. the
vector htat i use is pUASP vector.. i will be trying
it agian but it 
would
be good to know of any possible reasons where i may
have made a
mistake..it would be great to have a detailed protocol
from someone who
has done it and got transformants.. 
thats it..

Srikanth

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