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Hampton Carson hampton at hawaii.edu
Sun Jan 21 15:58:17 EST 2001


>From: Hampton Carson <hampton at hawaii.edu>
>Subject: Re: MOLD PROBLEM
>Cc: <bionet-drosophiila at net.bio.net>
>
>Dear Gregory:
>
>A simple solution is to collect a good representation from each mouldy
>culture (10-20 pairs of healthy young flies) Put together in on ordinary
>sterile glass vial with small medium. Change repeatedly to a new vial every
>hour for a day and laater for three hours (and overnight) for about two
>days and then introduce the adults into a fresh, sterile culture bottle and
>discard the rest of the old culture bottles.
>
>This uses the principle of "serial dilution" to get rid of the
>contamination.  It also works well to rid cultures of mites.  It uses up a
>lot of vials that have to be washed and sterilized, but the flies will
>efficiently clean themselves of spores fairly rapidly, dropping spores
>continually in the vials.  You can experiment with the number of transfers
>necessary, and possibly reduce the number of transfers that I have
>suggested.
>
>Good luck!
>
>Hampton Carson
>
>
>
>>    I am having a horrible problem with mold contamination  of my
>>cultures!  What would be the best method of getting rid of this  problem,
>>save dumping all my cultures and starting from scratch...which I really
>>don't want to do!!!  Any reply would be appreciated!   Thanks!
>>
>>Gregory M.    Spencer
>>
>>University at Albany
>>
>>Neurobiology Research    Center
>>
>>Department of Biological    Sciences
>>
>>1400 Washington Avenue
>>
>>Albany, New York 12222
>>
>>
>>
>>E-mail: gs5849 at albany.edu
>>
>>Phone: (518) 442-4335
>>
>>Fax: (518)  442-4767
>
>
>Hampton L. Carson, Professor Emeritus
>Department of Cell and Molecular Biology
>University of Hawaii.
>Home Address: 1314 Kalakaua Ave. Apt. 1111
>Honolulu, HI 96826
>

Hampton L. Carson, Professor Emeritus
Department of Cell and Molecular Biology
University of Hawaii.
Home Address: 1314 Kalakaua Ave. Apt. 1111
Honolulu, HI 96826



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